BNL CL.2 小鼠胚胎肝細胞

ATCC® Number:  TIB-73?       
Designations:  BNL CL.2 
Depositors:   P Patek, J Collins, M Cohn 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Mus musculus (mouse) 
Morphology:
 
Source: Organ: liver
Strain: BALB/c
Disease: normal
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Tumorigenic: No 
Age:  embryo 
Comments: The line was selected to grow in medium containing ornithine and phenylalanine in place of arginine and tyrosine.
Tested and found negative for ectromelia virus (mousepox).
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
 
Subculturing:  Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for 1 minute, remove trypsin and let the culture sit at room temperature until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Related Products: derivative:ATCC TIB-74
References: 22905: Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934