Daudi 人B細胞瘤細胞
ATCC® Number: CCL-213?
Designations: Daudi
Depositors: G Klein
Isotype: IgM
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast
Source: Organ: peripheral blood
Disease: Burkitt's lymphoma
Cell Type: B lymphoblast;
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Isolation: Isolation date: May, 1967
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Receptors: complement, expressed
Fc, expressed
Tumorigenic: Yes
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,12
D16S539: 10,12
D5S818: 8,13
D7S820: 8,10
THO1: 6,7
TPOX: 8,11
vWA: 15,17
Cytogenetic Analysis: Male human karyotype with stemline number of 46. The karyotype is diploid in 66% of the cells and is stable within the stemline.
Isoenzymes: G6PD, B
Age: 16 years
Gender: male
Ethnicity: Black
Comments: The Daudi line was derived from a 16-year-old Black male with Burkitt's lymphoma by E. Klein and G. Klein in May, 1967. The cells are negative for beta-2-microglobulin. They are positive for EBNA, VCA and Surface immunoglobulin (sIg+).The line carries Epstein-Barr virus. The Daudi is a well characterized B lymphoblast cell line which has been employed extensively in studies of mechanisms of leukemogenesis.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 to 5 X 10(5) viable cells/ml.
Interval: Maintain cell density between 3 X 10(5) and 2 to 3 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22550: Ohsugi Y, et al. Tumorigenicity of human malignant lymphoblasts: comparative study with unmanipulated nude mice, antilymphocyte serum-treated nude mice, and X- irradiated nude mice. J. Natl. Cancer Inst. 65: 715-718, 1980. PubMed: 6932523
23017: Klein E, et al. Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res. 28: 1300-1310, 1968. PubMed: 4174339
26046: Huber C, et al. Surface receptors on human haematopoietic cell lines. Clin. Exp. Immunol. 25: 367-378, 1976. PubMed: 963908
26047: Nilsson K, et al. Tumorigenicity of human hematopoietic cell lines in athymic nude mice. Int. J. Cancer 19: 337-344, 1977. PubMed: 14896
28315: Gao Y, et al. Induction of an exceptionally high-level, nontranslated, Epstein-Barr virus-encoded polyadenylated transcript in the uurkitts lymphoma line Daudi. J. Virol. 71: 84-94, 1997. PubMed: 8985326
32286: Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019
32830: Yamaguchi Y, et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed: 8943055
33091: Lewis JA, et al. Inhibition of mitochondrial function by interferon. J. Biol. Chem. 271: 13184-13190, 1996. PubMed: 8662694
33115: Montoya JG, et al. Human CD4+ and CD8+ T lymphocytes are both cytotoxic to Toxoplasma gondii-infected cells. Infect. Immun. 64: 176-181, 1996. PubMed: 8557337
