CCL-153 HFL1 人肺成纖維細胞
ATCC® Number:  CCL-153?      
Designations:  HFL1 
Depositors:   RG Crystal 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Homo sapiens (human) 
Morphology: fibroblast

 
Source: Organ: lung
Disease: normal
Cell Type: fibroblast
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Reverse Transcript: negative 
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9,11
D5S818: 12
D7S820: 9,10
THO1: 7,9
TPOX: 6,9
vWA: 17
Cytogenetic Analysis: normal human; diploid; stable
Isoenzymes:  G6PD, B
Age:  fetus 
Ethnicity:  Caucasian 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:  Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation:  culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
References: 23400: Breul SD, et al. Control of collagen production by human diploid lung fibroblasts. J. Biol. Chem. 255: 5250-5260, 1980. PubMed: 7372635