CRL-2065 MLTC-1 小鼠睪丸間質細胞瘤細胞
ATCC® Number:  CRL-2065?       
Designations:  MLTC-1 
Depositors:   RV Rebois 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent, Cells form islands that dome and the monolayer may not become confluent.
Organism: Mus musculus (mouse) 
Morphology: epithelial

 
Source: Organ: testis
Strain: C57BL/6
Disease: Leydig cell tumor
Cell Type: Leydig cell;
Cellular Products: progesterone [22276] 
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Applications: transfection host (Roche FuGENE® Transfection Reagents)
Receptors: chorionic gonadotropin; luteinizing hormone (LH) [22276]
Tumorigenic: Yes 
Cytogenetic Analysis: polyploid; modal number = 95 [22276]
Gender:  male 
Comments: This line was established from the M548OP transplantable Leydig cell tumor carried in C57BL/6 mice. [22276]
The cells retain the hormonal responsiveness of the original tumor.
Membrane adenyl cyclase activity is stimulated by treatment with human chorionic gonadotropin (hCG), luteinizing hormone, cholera toxin, sodium fluoride and guanyl-5'-ylimidodiphosphate, and produce progesterone in response to hCG. [22276]
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:  Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation:  Culture medium, 95%; DMSO, 5%
Doubling Time:  35 to 40 hrs 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22276: Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740