CRL-1432 NAMALWA 人Burkitt's 瘤細(xì)胞
ATCC® Number: CRL-1432?
Designations: NAMALWA
Depositors: NB Finter
Isotype: IgM; lambda light chain
Biosafety Level: 2 [Cells Contain Herpesvirus ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Cellular Products: immunoglobulin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche FuGENE® Transfection Reagents)
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 7,9.3
TPOX: 6,11
vWA: 14
Cytogenetic Analysis: This is a human cell line with the hypodiploid chromosome count. The modal chromosome number was 44, occurring in 30% of cells. The rate of cells with higher ploidies was 2.8%. Twelve to 14 marker chromosomes were common to most cells. Among these were ins(1;?) (q11;?), ins(3) (q11q29), del(3) (p12/13), der(6)t(3;6) (p21;p25), t(15q21q) and seven to nine others. Normal N3 was absent, and N7 had three copies per cell. The X chromosome was single. There was no Y chromosome.
Age: 3 years
Gender: female
Comments: The cells contain the Epstein-Barr virus (EBV) genome.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 92.5%; fetal bovine serum, 7.5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Cultures should be started at 5 X 10(5) viable cells/ml and subcultured at 2 X 10(6) cells/ml.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1059: Klein F, et al. Large-scale production and concentration of human lymphoid interferon. Antimicrob. Agents Chemother. 15: 420-427, 1979. PubMed: 464569
1093: Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763
22364: Klein G, et al. Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus. Int. J. Cancer 10: 44-57, 1972. PubMed: 4122458
58035: Nyormoi O, et al. Sensitivity to EBV superinfection and IUdR inducibility of hybrid cells formed between a sensitive and a relatively resistant Burkitt lymphoma cell line. Int. J. Cancer 12: 396-408, 1973. PubMed: 4365093
ATCC® Number: CRL-1432? Price: $318.00
Designations: NAMALWA
Depositors: NB Finter
Isotype: IgM; lambda light chain
Biosafety Level: 2 [Cells Contain Herpesvirus ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast
Source: Disease: Burkitt's lymphoma
Cell Type: B lymphocyte;
Cellular Products: immunoglobulin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host (Roche FuGENE® Transfection Reagents)
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 7,9.3
TPOX: 6,11
vWA: 14
Cytogenetic Analysis: This is a human cell line with the hypodiploid chromosome count. The modal chromosome number was 44, occurring in 30% of cells. The rate of cells with higher ploidies was 2.8%. Twelve to 14 marker chromosomes were common to most cells. Among these were ins(1;?) (q11;?), ins(3) (q11q29), del(3) (p12/13), der(6)t(3;6) (p21;p25), t(15q21q) and seven to nine others. Normal N3 was absent, and N7 had three copies per cell. The X chromosome was single. There was no Y chromosome.
Age: 3 years
Gender: female
